Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin
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چکیده
منابع مشابه
Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin.
Vol. 40, No. 5 (2006) The binding of avidin to biotin is the strongest known noncovalent biological interaction [dissociation constants (Kd) approximately 10 -15 M] between a protein and ligand (1). The high affinity and specificity of the avidin-biotin interaction is utilized for enriching biotin-containing proteins from mixtures (2,3). Water-soluble Nhydroxysulfosuccinimide (sulfo-NHS) deriva...
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A biotinylated nucleotide analog containing a disulfide bond in the 12-atom linker joining biotin to the C-5 of the pyrimidine ring has been synthesized. This analog, Bio-SS-dUTP, is an efficient substrate for Escherichia coli DNA polymerase I. Bio-SS-dUTP supported DNA synthesis in a standard nick-translation reaction at 35%-40% the rate of an equal concentration of the normal nucleotide, TTP....
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2006
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112171